NAD+ 500mg Dosing
Ensure that you sterilise the cartridge stopper with an alcohol swab before connecting the needle to the pen.
1. Prime the pen to remove any trapped air by turning the dial in increments of 5 to 10 units and pressing the button, repeat until a small drop of liquid appears at the tip of the needle.
2. Adjust the dose on the window to the recommended starting dose. Double check your dose before getting ready to insert the needle.
3. First sterilise the area to be injected with an alcohol swab. Pinch the skin on the area you plan to inject (usually subcutaneously in the abdominal area) between your fingers, then insert the needle at a 90 degree angle to the full length of the needle, hold in place then press the button on the pen to fully dispense the peptide solution. Hold the needle in place for 5 to 10 seconds to allow the solution to diffuse into the tissue.
NAD+ 500mg Dosing Recommendation
NEVER SHARE YOUR NEEDLES OR PEN WITH ANYONE.
Keep Refridgerated at 2°
Ensure you sterilise the cartridge stopper first by wiping the rubber port with an alcohol swab- do not touch the stopper once sterilised. Do not touch opened needles. Use a new needle for each injection. If you are reusing needles, wipe the needle tip with an alcohol swab before using.
Where to Inject: Inject SubQ into belly fat
Dosing:
| Week | Units per injection | Frequency | Injection days |
|---|---|---|---|
| Week 1 | 15 units | 2x per week | Mon & Thu |
| Week 2 | 15 units | 2x per week | Mon & Thu |
| Week 3 | 18 units | 2x per week | Mon & Thu |
| Week 4 | 21 units | 3x per week | Mon, Wed, Fri |
| Week 5 | 21 units | 3x per week | Mon, Wed, Fri |
| Week 6 | 24 units | 3x per week | Mon, Wed, Fri |
Best Time to Inject
| Time | Recommendation |
|---|---|
| Morning | ✅ Best — energy, focus, alertness |
| Early afternoon | ✅ Acceptable |
| Evening | ❌ Avoid — may cause insomnia |
Do's
| Do | Why |
|---|---|
| Start low (25mg) | Assess tolerance before increasing |
| Increase dose gradually | Gradual adaptation reduces side effects |
| Inject in the morning | Avoids insomnia |
| Inject slowly | Reduces stinging/burning |
| Warm vial slightly before injection | Roll in hands — reduces stinging |
| Stay hydrated | Supports NAD+ function |
| Refrigerate vial | Maintains stability |
| Rotate injection sites | Prevents lumps |
Don'ts
| Don't | Reason |
|---|---|
| Don't skip the low starting dose | May cause nausea, headache, flushing |
| Don't inject in the evening | May cause insomnia |
| Don't exceed 40mg per injection (on 500mg vial) | No added benefit at this concentration |
| Don't use if pregnant or breastfeeding | No safety data |
| Don't use if solution is cloudy | Means it's degraded |
| Don't freeze the vial | Freezing damages the molecule |
⚠️ Common Side Effects (Starting Low Minimizes These)
| Side Effect | How Common at low dose | What To Do |
|---|---|---|
| Injection site stinging | Common (30-40%) | Inject slowly, warm vial first |
| Nausea | Uncommon (5-10% at low dose) | Inject after a small meal |
| Headache | Uncommon (5-10% at low dose) | Hydrate, lower dose |
| Fatigue | Uncommon (5-10% at low dose) | Usually passes after 1-2 doses |
| Insomnia | Rare (if morning injection) | Switch to earlier morning |
⚠️ MEDICAL DISCLAIMER: This information is for educational and research purposes only and does not constitute medical advice. Peptides should only be used under the supervision of a qualified healthcare provider. Do not use this information to self-diagnose or self-treat any medical condition. Always consult a doctor before starting any peptide or supplement protocol.